Homobifunctional NHS esters

 

Product No. Name Pkg Price
 
57841

(BSOCOES) (Molbio Ultra, 99+%)
Syn:  bis[2-(Succinimidooxycarbonyloxy)ethyl]sulfone
C14H16N2O12S; Mr=436.35; mp 158 °C
Spacer Arm length:  13.0 Å.

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● Amine reactive homobifunctional crosslinking reagent.1
 ● Used to crosslink b-endorphin to opioid receptors.2
 ● Base cleavable; pH 11.6, 37 ºC/2 h.1
 ● For purification of calcitonin receptors.3

1. Zarling, D.A., Watson, A., Bach, F.H., (1980) J. Immunol. 124, 913-920.
2. Howard, A.D., et.al. (1985) J. Biol. Chem. 260, 10833-18039.
3. Bouizar, Z., et.al. (1986) Eur. J. Biochem. 155, 141-147.

Storage 0-4 °C

 100mg















 $71.50
57903







 Succinimidyloxycarbonyl-b-alanine-hydroxysuccinimide ester
C12H13N3O8; Mr=327.25;  mp  163-164 °C

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● Homobifunctional NHS ester.


Storage 0-4 °C.

 250mg







 $110.00
57344
















 (DST), (Molbio Ultra, 99+%)
Disuccinimidyl-L-tartrate
C12H12N2O10; Mr=344.23; mp 168 °C (Decomp)
Spacer Arm length:  6.4 Å.

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● Amino reactive homobifunctional crosslinking reagent for protein crosslinking.
 ● Crosslinking the cell surface receptor of CSF-2a.1
 ● Crosslinking of cytochrome C reductase.2
 ● Studing ATP transport in E. coli.3
 ● Croslinking to cellular membranes.4
 ● Cleavable by 0.015 M NaIO4, or by 0.1 % SDS, 0.02 M NaHPO4, pH 7.0/2h.2

1. Park, L. S., Friend, D., Gillis, S., Urdal, D.L., (1986) J. Biol. Chem. 261, 205-210.
2. Smith, R. J., Capaldi, R.A., Muchmore, D., Dahlquist, F., (1978) Biochem. 17, 3719-3723.
3. Bragg, P.D., Hou, C., (1980) Eur. J. Biochem. 106, 495-503.
4. Farries, T.C., Atkinson, J.P. (1989) J. Immunol. 142, 842-847.

Storage 0-4 °C.

 50mg 
















 $125.00
57345







 Di-sulfo-succinimidyl-L-tartrate
C12H10N2S2O16Na2;   Mr=548.34
Spacer Arm length:  6.4 Å.

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 50mg







 $195.00
57439 




















 (DSS) (Molbio Ultra, 99+%)
Disuccinimidyl suberate
[68528-80-3]; C16H20N2O8; Mr=368.34; mp 169-170 °C
Spacer Arm length:  11.4 Å.

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● Amino reactive homobifunctional crosslinking reagent for protein crosslinking.1
 ● Widely used biochemical probe.
 ● Conjugating hINF-g to cell surface receptors.2
 ● Crosslinking HCG to its receptor.3
 ● Crosslinking IL-2 to cell surfaces.4
 ● Crosslinking CSF-1 to cell surface receptors.5
 ● Crosslinking angiotensin II to its receptor.6

1. Tsudo, M., et.al. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 4215-4218.
2. Novick, D., Orchansky, P., Revel, M., Rubenstein, M. (1987) J. Biol. Chem. 262, 8483-8487.
3. Rebois, R.V., Omedeo-Sale, F., Fishman, P.H., (1981) Proc. Natl. Acad. Sci. U.S.A. 78, 2086-2089.
4. Cox, G.W., Mattieson, B.J., Giardina, S.L., Varesio, L. (1990) J. Immunol. 145, 1719-1726.
5. Morgan, C.J., Stanley, E.R., (1984) Biochem. Biophys. Res. Comm. 119, 35-41.
 6. Sen, I., Bull, H.G., Sutter, R.I. (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 1697-1683.

Storage 0-4 °C

 1.0g




















 $59.00
57424










 Disuccinimidyl sebacate
C18H24N2O8; Mr=396.39; mp 150-151 °C

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● Crosslinking ligand-receptor complexes of CSF-1.1
 ● Conjugating vasoactive intestinal peptide to its receptor.2

 1. Morgan, C.J., Stanley, E.R. (1984) Biochem. Biophys. Res. Commun. 119, 35-41.
2. Wood, C.L., O'Dorisio, M.S. (1985) J. Biol. Chem. 260, 1243-1247.

Storage 0-4 °C.

 1.0g










 $82.50
57951










 (DSD)
Disuccinimidyl dodecanoate
C20H28N2O8; Mr=424.45; mp  156-157 °C

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● Amino reactive homobifunctional crosslinking reagent.1
 ● Extended 12-atom spacer.

 1. Tsudo, M., et.al. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 4215-4218.

Storage 0-4 °C.

 1.0g










 $82.50
57641



















(DTSP)  99 %
Dithio-bis-succinimidyl propionate (Lomant's Reagent)
[57757-57-0]; C14H16N2S2O8;   Mr=404.42; mp  128-132 °C
Spacer Arm length:  12.0 Å.

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● Amino reactive, homobifunctional and reversible crosslinking reagent.1,2
 ● Cleavable with 10-50 mM DTT, 37 ºC/30 min.
 ● Cleavable with TCEP, pH 5.0/5 min.6
 ● Useful for characterizing CSF-2a receptors.3
 ● Crosslinking antigens to monoclonal antibodies.4
 ● Identification of receptors for VIP.2
 ● Studing glycoproteins in human RBC.5

 1. Lomant, A.J., Fairbanks, G. (1976) J. Mol. Biol. 104, 243-261.
2. Laburthe, M., Breant, B., Rouyer-Fessard, C. (1984) Eur. J. Biochem. 139, 181-187.
3. Park, L.S., Friend, D., Gillis, S., Urdal, D.L. (1986) J. Biol. Chem. 261, 205-210.
4. Hamada, H., Tsuro, T. (1987) Anal. Biochem. 160, 483-488.
5. Schweizer, E., Angst, W., Lutz, H.V., (1982) Biochem. 21, 6807-6818.
6. Han, J.C., Han, G.Y. (1994) Anal. Biochem. 220, 5-10.

Storage 0-4 °C

 1.0g



















 $125.00
57212










 Hexadecanediol-bis-succinimidyl carbonate
C26H40N2O10; Mr=540.61

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● Extended spacer arm. Cleavable!
 ● Dimeric adducts are cleaved with 0.1 M hydroxylamine, phosphate buffer, pH 8.1.

Storage 0-4 °C

 100mg










 $125.00
57428



















 (BS3)
bis-sulfo-succinimidyl suberate
[82436-77-9]; C16H18N2S2O14Na2; Mr=572.43; mp >300 °C
Spacer Arm length:  11.4 Å.

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● Highly versatile protein crosslinking reagent.1
 ● Water soluble and membrane impermeable due to sodium sulfate groups.2
 ● Used to study anion exchange in human erythrocytes.3
 ● Useful for crosslinking insulin to its receptor.4
 ● Covalently crosslinking b-endorphin to calmodulin.5
 ● Used for crosslinking subunits of cytochrome b559.6

 1. Souza, E.D., et.al. (1988) J. Biol. Chem. 263, 3943-3951.
2. Staros, J.V. (1982) Biochem. 21, 3950-3955.
3. Staros, J.V., Kakkad, B.P., (1983) J. Membrane Biol. 74, 247-2254.
4. Waugh, S.M., DiBella, E.E., Pilch, P.F., (1989) Biochem. 28, 3448-3455.
5. Giedroc, D.P., Keravis, T.M., Staros, J.V., Ling, N., Wells, J.N., Puett, D. (1985) Biochem. 24, 1203-11.
6. Knoller, S., Shpungin, S., Pick, E. (1991) J. Biol. Chem. 266, 2795-2804.

Storage 0-4 °C

 100mg



















 $82.50