p-Azidobenzoic acid (99+%)
C₇H₅N₃O₂; M_r=163.14

● Useful for generating photoaffinity probes for mapping receptor-ligand complexes.¹
● Introduces carboxylate functionality onto non-derivatizable surfaces, ie. polystyrene, polypropylene; microtiter plates, beads, etc.

1. Chatreneet, B., et.al. (1990) Proc. Natl. Acad. Sci. USA 87, 3378.

Storage 25 °C.

● Amino and photoreactive protein crosslinking reagent.¹
● Photoaffinity crosslinking of calmodulin to binding proteins.^2,3

1. Vandlan, R.L., et.al. (1985) J. Biol. Chem. 260, 10889-10892.
2. Wood, C.L., et.al. (1985) J. Biol. Chem. 260, 1243-1247.
3. Goewert, R.R., et.al. (1982) Biochemistry 21, 5310.

Storage 0-4 °C.

● Photoreactive heterobifunctional crosslinking reagent.¹
● Useful for crosslinking glycoproteins to antibodies.²

1. O'Shannessy, D.J., et.al. (1985) J. Applied Biochem. 7, 347-355.
2. O'Shannessy, D.J., et.al. (1984) Immunol. Lett. 8, 273-277.

Storage 0-4 °C.

● Fluorine groups stabilize intermediate nitrene, leading to higher yields of intermolecular insertion products.¹
● Surface modification reagent for derivatizing unactivated surfaces; i.e. polystyrene, polypropylene, metal films, glass, DNA, RNA, etc.

1.  Keana J. F. W., Cai, S. X. (1990) J. Org. Chem. 55, 3640-3647.

Storage 0-4 °C.

● Water soluble!
● Fluorine groups stabilize intermediate nitrene, leading to higher yields of intermolecular insertion products.¹

1.  Keana J. F. W., Cai, S. X. (1990) J. Org. Chem. 55, 3640-3647.

Storage 0-4 °C.

● Arginine and photoreactive heterobifunctional crosslinking reagent.¹
● Irreversibly modifies arginine side chain residues at pH 7.0-8.0.  Used to crosslink RNA-protein interactions.²

1. Sgro, J., et.al. (1984) Eur. J. Biochem. 154, 69-76.
2. Poltz, S.M., Noller, H. F., McWhirter, P. D. (1981) Biochemistry 20, 372-378.

Storage 0-4 °C.

● Carbonyl and photoreactive crosslinking reagent.
● Immobilization of glycoproteins through their carbohydrate residues.

Storage 0-4 °C.

● Heterobifunctional crosslinking reagent for photoaffinity labeling.^1,2

1. Hagedorn, M., et.al. (1978) J. Org. Chem. 43, 2070-2072.
2. Tometsko, A.M., et.al. (1976) Int. J. Peptide Protein Res. 8, 331.

Storage 0-4 °C.

● Sulfhydryl and photoreactive crosslinking reagent.¹

1. Schwartz, I., Ofengand, J. (1974) Proc. Natl. Acad. Sci. 71, 230.

Storage 0-4 °C.

● Amino and photoreactive heterobifunctional crosslinker.^1,2
● Photolysis occurs at 320-350 l.  Labeling is usually complete within 15-20 min.

1. Schafer, H. (1987) Chemical Modification of enzymes, ED. Eyzaguirre, J. 45-62.
2. Krieg, U.C., Walter, P., Johnson, A.E. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 8604-8608.

Storage 0-4 °C.

● Water soluble and extended analog of ANB-NOS!
● Photolysis occurs at 320-350 l.  Labeling is usually complete within 15-20 min.

Storage 0-4 °C.

● Converts amines into photoaffinity probes, or surface modification reagents.

Storage 0-4 °C.

● Converts sulfhydryls into cleavable photoaffinity probes.
● Used to label granulocyte C5a receptor.¹

1. Johnson, R.J., Chenoweth, D.E. (1985) J. Biol. Chem. 7161-7164.

Storage 0-4 °C.

● Water soluble!
● Heterobifunctional crosslinking reagent, Photolysis occurs at 320-350 nm.¹

1. Schmitt, M., et.al. (1983) J. Biol. Chem. 258, 649-654.

Storage 0-4 °C.

(SAND) 99 %
sulfo-Succinimidyl 2-[m-azido-o-nitrobenzamido]ethyl-1,3´-dithiopropionate
C₁₆H₁₅N₆O₁₀S₃Na;  M_r=570.51
Spacer Arm Length:  18.5 Å.

● Water soluble!
● Heterobifunctional crosslinking reagent, Photolysis occurs at 320-350 nm.¹
● Long chain and cleavable analog of ANB-NOS.  Conjugated products can be cleaved with mild reducing reagents, i.e. DTT, DTE, TCEP.

Storage 0-4 °C.